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human bladder cancer derived cell lines rt112  (DSMZ)


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    DSMZ human bladder cancer derived cell lines rt112
    Human Bladder Cancer Derived Cell Lines Rt112, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+bladder+cancer+cell+line+rt112/RT-112/pm36944729-294-8-21
    Average 95 stars, based on 155 article reviews
    human bladder cancer derived cell lines rt112 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Expressing:

    Article Title: MKP-1 overexpression is associated with chemoresistance in bladder cancer via the MAPK pathway
    Article Snippet: The human bladder cancer cell line RT112 was purchased from the Leibniz Institute DSMZ and maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere containing of 5% CO 2 .. Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Transfection:

    Article Title: MKP-1 overexpression is associated with chemoresistance in bladder cancer via the MAPK pathway
    Article Snippet: The human bladder cancer cell line RT112 was purchased from the Leibniz Institute DSMZ and maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere containing of 5% CO 2 .. Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Reverse Transcription:

    Article Title: MKP-1 overexpression is associated with chemoresistance in bladder cancer via the MAPK pathway
    Article Snippet: The human bladder cancer cell line RT112 was purchased from the Leibniz Institute DSMZ and maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere containing of 5% CO 2 .. Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Real-time Polymerase Chain Reaction:

    Article Title: MKP-1 overexpression is associated with chemoresistance in bladder cancer via the MAPK pathway
    Article Snippet: The human bladder cancer cell line RT112 was purchased from the Leibniz Institute DSMZ and maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere containing of 5% CO 2 .. Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Microscopy:

    Article Title: MKP-1 overexpression is associated with chemoresistance in bladder cancer via the MAPK pathway
    Article Snippet: The human bladder cancer cell line RT112 was purchased from the Leibniz Institute DSMZ and maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere containing of 5% CO 2 .. Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Western Blot:

    Article Title: MKP-1 overexpression is associated with chemoresistance in bladder cancer via the MAPK pathway
    Article Snippet: The human bladder cancer cell line RT112 was purchased from the Leibniz Institute DSMZ and maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere containing of 5% CO 2 .. Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Control:

    Article Title: MKP-1 overexpression is associated with chemoresistance in bladder cancer via the MAPK pathway
    Article Snippet: The human bladder cancer cell line RT112 was purchased from the Leibniz Institute DSMZ and maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere containing of 5% CO 2 .. Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Negative Control:

    Article Title: MKP-1 overexpression is associated with chemoresistance in bladder cancer via the MAPK pathway
    Article Snippet: The human bladder cancer cell line RT112 was purchased from the Leibniz Institute DSMZ and maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere containing of 5% CO 2 .. Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Small Interfering RNA:

    Article Title: MKP-1 overexpression is associated with chemoresistance in bladder cancer via the MAPK pathway
    Article Snippet: The human bladder cancer cell line RT112 was purchased from the Leibniz Institute DSMZ and maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere containing of 5% CO 2 .. Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Activity Assay:

    Article Title: MKP-1 overexpression is associated with chemoresistance in bladder cancer via the MAPK pathway
    Article Snippet: The human bladder cancer cell line RT112 was purchased from the Leibniz Institute DSMZ and maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere containing of 5% CO 2 .. Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Sulforhodamine B Assay:

    Article Title: MKP-1 overexpression is associated with chemoresistance in bladder cancer via the MAPK pathway
    Article Snippet: The human bladder cancer cell line RT112 was purchased from the Leibniz Institute DSMZ and maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere containing of 5% CO 2 .. Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    CCK-8 Assay:

    Article Title: MKP-1 overexpression is associated with chemoresistance in bladder cancer via the MAPK pathway
    Article Snippet: The human bladder cancer cell line RT112 was purchased from the Leibniz Institute DSMZ and maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere containing of 5% CO 2 .. Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).Tissue culture media and serum were purchased from Hyclone (GE Healthcare Life Sciences).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Cell culture Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).

    Article Title: SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models
    Article Snippet: Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).Human lung cancer cell line NCI-H1581, human acute myelogenous leukemia cell line KG1, human gastric cancer cell lines SNU16 and KATOIII, and human bladder cancer cell line NCI-H716 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Human bladder cancer cell line RT112 was purchased from the DSMZ-German collection of microorganisms and cell cultures.. Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).Human bladder cancer cell line UMUC14 was purchased from the European Collection of Cell Cultures (ECACC).



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    95
    DSMZ human bladder cancer cell line rt112
    MKP-1 and MAPK expression in <t>RT112</t> cells transfected with NC and MKP-1 siRNA. (A) Relative MKP-1 expression in the siNC and siMKP-1 groups was examined using reverse transcription-quantitative PCR. **P<0.01. (B) Representative microscopic images of siNC and siMKP-1 treated cells captured in both 2D and 3D environments under a phase contrast microscope. (C) MKP-1 expression of the siNC and siMKP-1 groups, as determined via western blotting. GAPDH was used as the internal control. (D) Phosphorylated and total ERK1/2 protein expressions of the siNC and siMKP-1 group, as determined via western blotting. GAPDH was used as the internal control. (E) Phosphorylated and total p38 protein expressions of the siNC and siMKP-1 group, as determined via western blotting. GAPDH was used as the internal control. (F) Phosphorylated and total JNK protein expression levels of the siNC and siMKP-1 group, as determined via western blotting. GAPDH was used as the internal control. MKP-1, mitogen activated protein kinase phosphatase-1; NC, negative control; siMKP-1, MKP-1 small interfering RNA; siNC, small interfering negative control.
    Human Bladder Cancer Cell Line Rt112, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MKP-1 and MAPK expression in RT112 cells transfected with NC and MKP-1 siRNA. (A) Relative MKP-1 expression in the siNC and siMKP-1 groups was examined using reverse transcription-quantitative PCR. **P<0.01. (B) Representative microscopic images of siNC and siMKP-1 treated cells captured in both 2D and 3D environments under a phase contrast microscope. (C) MKP-1 expression of the siNC and siMKP-1 groups, as determined via western blotting. GAPDH was used as the internal control. (D) Phosphorylated and total ERK1/2 protein expressions of the siNC and siMKP-1 group, as determined via western blotting. GAPDH was used as the internal control. (E) Phosphorylated and total p38 protein expressions of the siNC and siMKP-1 group, as determined via western blotting. GAPDH was used as the internal control. (F) Phosphorylated and total JNK protein expression levels of the siNC and siMKP-1 group, as determined via western blotting. GAPDH was used as the internal control. MKP-1, mitogen activated protein kinase phosphatase-1; NC, negative control; siMKP-1, MKP-1 small interfering RNA; siNC, small interfering negative control.

    Journal: Oncology Letters

    Article Title: MKP-1 overexpression is associated with chemoresistance in bladder cancer via the MAPK pathway

    doi: 10.3892/ol.2020.11741

    Figure Lengend Snippet: MKP-1 and MAPK expression in RT112 cells transfected with NC and MKP-1 siRNA. (A) Relative MKP-1 expression in the siNC and siMKP-1 groups was examined using reverse transcription-quantitative PCR. **P<0.01. (B) Representative microscopic images of siNC and siMKP-1 treated cells captured in both 2D and 3D environments under a phase contrast microscope. (C) MKP-1 expression of the siNC and siMKP-1 groups, as determined via western blotting. GAPDH was used as the internal control. (D) Phosphorylated and total ERK1/2 protein expressions of the siNC and siMKP-1 group, as determined via western blotting. GAPDH was used as the internal control. (E) Phosphorylated and total p38 protein expressions of the siNC and siMKP-1 group, as determined via western blotting. GAPDH was used as the internal control. (F) Phosphorylated and total JNK protein expression levels of the siNC and siMKP-1 group, as determined via western blotting. GAPDH was used as the internal control. MKP-1, mitogen activated protein kinase phosphatase-1; NC, negative control; siMKP-1, MKP-1 small interfering RNA; siNC, small interfering negative control.

    Article Snippet: The human bladder cancer cell line RT112 was purchased from the Leibniz Institute DSMZ and maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere containing of 5% CO 2 .

    Techniques: Expressing, Transfection, Reverse Transcription, Real-time Polymerase Chain Reaction, Microscopy, Western Blot, Control, Negative Control, Small Interfering RNA